Table 2. Thermodynamic parameters of holo-LA in the presence of varying calcium chloride concentrations under dilute and crowded conditionsa.
[CaCl2] | Dilute | Crowded (400 g/l Ficoll 70) | ||
(mM) | T m (°C) | ΔH m (kcal mol−1) | T m (°C) | ΔH m (kcal mol−1) |
0.0 | 54.4 | 53 | ND | ND |
0.1 | 60.2 | 64 | ND | ND |
1.0 | 65.3 | 72 | ND | ND |
5.0 | 66.6 | 74 | ND | ND |
10.0 | 68.2 | 77 | ND | ND |
25.0 | 68.3 | 79 | ND | ND |
50.0 | 68.8 | 80 | ND | ND |
100.0 | 69.0 | 78 | 68.8 | 76 |
200.0 | 68.2 | 78 | 68.3 | 80 |
400.0 | 68.5 | 77 | 68.6 | 79 |
Errors in T m and ΔH m are 0.3–0.8% and 5–8%, respectively.
Not determined.