Figure 4. The feasibility of the synthesized FVP-B and our developed assay.

(A) 228 nM purified LC/B was mixed with 8.4 µM FVP-B in reaction buffer to a final volume of 500 µl. Reaction was incubated at 37°C and the fluorescent intensity was measured in quartz cuvette every 30 min. The total duration of measurement was 8 h. The data were processed by Origin85. (B) The fluorescent intensity peak at 502 nm was selected to represent the trend of the whole data. The data were processed with Excel.