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. 2014 Dec 1;9(12):e114124. doi: 10.1371/journal.pone.0114124

Figure 5. Optimization of the developed assay system.

Figure 5

To optimize the assay and test the sensitivity of our detection assay, ten-fold dilution of LC/B was performed with the concentration of FVP-B fixed at 8.4 µM (A); five-fold dilution of FVP-B, with the LC/B concentration fixed at 22.8 nM (B); the fluorescent intensity change at 502 nm of further diluted LC/B to 22.8 pM with 1.68 µM FVP-B and extended incubation time (C); and a spectrum representative of 22.8 pM LC/B incubated with 1.68 µM FVP-B for extended time span (D). Viewing from the data, the detection sensitivity of the developed assay is about 22.8 nM of LC/B within 30 min, but the sensitivity can be improved to 22.8 pM of LC/B with 2 h incubation. The data were processed by Origin85 and Excel.