Figure 6. Limit of detection of the developed LC/B detection system.

Serial LC/B concentrations were incubated with 8.4 µM FVP-B in 500 µl reaction volume for 1 h incubation at 37°C. The fluorescence intensities were potted versus the concentration of LC/B. For each concentration, at least five replicates were carried out. LOD  = 3*S/k (S means standard deviation of negative control, k means slope).