Figure 7. The inhibitory effect of EDTA on LC/B.

228 nM of LC/B was mixed with 8.4 µM FVP-B with reaction buffer to a final volume of 500 µl, incubated at 37°C for 1 h, and then the fluorescent intensity was measured by a Fluorescence Spectrometer in quartz cuvette (A); 100 µl reaction volume, which contained 228 nM LC/B with 8.4 µM FVP-B, were carried out in 96 well plate after 1 h incubation at 37°C (B). For simplicity, the fluorescent intensity peak at 502 nm was selected. The data obtained were generated from at least three times repeats, and then processed with Excel and GraphPad Prism, with the negative control data subtracted.