Figure 4. Effects of sgRNA targeting cyclin D1 on viable cell numbers and cell proliferation.

HSC-3 cells were plated at 1×10⁵ cells/well in 24 well dishes, and then cultured for 24 h. sgHT2 or sgH5 was added at 200 nM and then cultured for a further 24 h. Cisplatin (10 µM) or vehicle was added to cells, after which the cells were cultured for 72 h. The living cell numbers were quantitated as described in Materials and Methods (A). Cell proliferation was measured by BrdU incorporation using an ELISA kit (B). The relative living cell numbers and BrdU incorporation in the absence of both sgRNAs and cisplatin are adjusted to 1. Each assay represents a separate experiment performed in triplicate. Data are means ± S.D. *P<0.05.