Figure 5. The effects of sgRNA targeting of cyclin D1 on cyclin D1 expression and cell proliferation of other human cancer cell lines and normal cells.
(A) Hela, MG-63, HEK293 and HSC-3 cells were plated at 1×105 cells/well in 24 well dishes, and cultured for 24 h. sgHT5, sgH5, H5470 (control, C) or vehicle (-) was added at 200 nM and then the cells cultured for another 24 h. Cyclin D1 mRNA level was determined by qRT-PCR. (B) Cell proliferation of HEK293 cells was measured by BrdU incorporation using an ELISA kit. The cyclin D1 mRNA level and BrdU incorporation in the absence of sgRNAs are adjusted to 1. Each assay represents a separate experiment performed in triplicate. Data are means ± S.D.