Table1. Sequence of sgRNAs.
Name | Sequence(5′ to 3′) |
HT1 | 5′-pGCAGCAGUGGCGCAAUGGAUAACGCGUUGUC-3′ |
HT2 | 5′-pCCCGCUGUGGCGCAAUGGAUAACGCGAUUGG-3′ |
HT3 | 5′-pGAUGUGCUGGCGCAAUAACGCGUGUCA-3′ |
HT4 | 5′-pGGCACCGUGGCGCAAUGGAUAACGCGGCCUC-3′ |
HT5 | 5′-pCGGCCUCUGGCGCAAUGGAUAACGCGCGCCA-3′ |
HT6 | 5′-pACACUUGUGGCGCAAUGGAUAACGCGUAAUA-3′ |
H2 | 5′-pCCCGCUG-3′ |
L2 | 5′-pCCCGCUGCCACCAU-3′ |
H5 | 5′-pCGGCCUC-3′ |