Table 4. Myostatin (MSTN) mRNA level and DNA methylation of gene exon 1 region.
Heavy | Light | P | ||||||
Item | CM | HM | CM | HM | SEM | HW | Met | HW × Met |
mRNA level | 1.00ab | 0.60b | 1.45a | 0.80b | 0.09 | 0.088 | 0.011 | 0.506 |
Methylation (%) | 33.3ab | 29.6ab | 20.7b | 43.5a | 2.5 | 0.900 | 0.079 | 0.020 |
Heavy −48.3±0.1 g; Light −41.7±0.1 g; CM – control methionine levels (0.50% and 0.43% methionine during 1–21 d and 22–42 d, respectively); HM - high methionine levels (0.60% and 0.53% methionine during 1–21 d and 22–42 d, respectively); HW - hatching weight.
SEM - standard error of the mean.
Within the same row, different superscripts indicate significant differences (P<0.05).