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. 2014 Dec 1;9(12):e114119. doi: 10.1371/journal.pone.0114119

Figure 6. AtNUDX8 expression peaks at eight hours and is repressed by SA.

Figure 6

qRT-PCR analysis of AtNUDX8 relative expression levels in the leaves of WT plants without SA treatment and 1 mM SA in a 24 hour time course expression analysis. Three-week-old plants were sprayed with 1 mM of SA and water (mock control), and samples were collected at different time points. Bars represent change in expression of AtNUDX8 transcript in WT plants without SA and SA-treated relative to the internal control ACTIN2 used as reference gene (since it does not vary under the different conditions and treatments tested). Relative expression values were normalized to ACTIN2 mRNA using the ΔΔCT method. Data are reported as means ±SD of three independent biological replicates. Asterisk indicates significant difference according to Student's t test (P<0.05). Zeitgeber time is indicated from the time the lights were turned on at 0 hour for a period of 24 hours.