Table 3. Expression of marker genes at 15 and 30 days post treatment (dpt).
Treatment | n | Gene | |||||||
tcrac | igu | bik | vasa | gsdf | inhα | amh | cyp19a1a | ||
Female 15 dpt | |||||||||
Control | 7 | ||||||||
Anti-Gsdf-a | 3 | 1.2 | −1.4 | −1.1 | 1.0 | −2.0 | 1.2 | 1.3 | 1.7 |
Anti-Gsdf-b | 3 | −1.5 | 2.1 | 1.3 | −1.6 | −1.4 | 1.0 | −1.1 | −1.6 |
Anti-Gdf9-a | 2 | 1.0 | 1.7 | 1.4 | −1.6 | −1.6 | −1.1 | 1.0 | 1.4 |
Anti-Gdf9-b | 6 | −1.9 | 2.6 | 1.8 | −2.1 | 1.5 | −1.3 | −1.1 | −1.2 |
Anti-Cd205 | 5 | −1.6 | 2.9 | 1.7 | −1.5 | 1.0 | 1.0 | 1.2 | −1.3 |
Male 15 dpt | |||||||||
Control | 6 | ||||||||
Anti-Gsdf-a | 3 | −1.9 | −1.6 | −1.3 | 1.4 | 1.2 | 1.7 | 1.7 | −1.3 |
Anti-Gsdf-b | 3 | −2.6 | 2.5 | −1.3 | 1.1 | −1.1 | 1.5 | 1.0 | −2.9 |
Anti-Gdf9-a | 3 | −3.0 | −1.4 | 1.0 | 1.7 | 1.0 | 1.5 | 1.5 | −3.3 |
Anti-Gdf9-b | 3 | 1.2 | 1.2 | 1.4 | −1.5 | −1.3 | −1.4 | −1.1 | −1.3 |
Anti-Cd205 | 4 | −2.1 | 1.0 | −1.3 | −2.0 | −1.3 | −1.4 | −1.2 | −2.2 |
Female 30 dpt | |||||||||
Control | 9 | ||||||||
Anti-Gsdf-a | 4 | 1.7 | 1.3 | −1.3 | 1.7 | −1.5 | 1.9 | 1.2 | 1.2 |
Anti-Gsdf-b | 4 | 1.1 | 1.2 | −1.7 | 1.2 | −1.2 | −1.3 | 1.0 | −2.7 |
Anti-Gdf9-a | 4 | −1.9 | 2.4 | 1.2 | −1.2 | −1.3 | 1.1 | −1.2 | 1.3 |
Anti-Gdf9-b | 6 | −1.4 | 2.2 | −1.2 | −1.3 | 1.5 | −1.8 | −1.7 | −2.3 |
Anti-Cd205 | 6 | −2.1 | 3.7 | 1.4 | −1.4 | 1.3 | −1.6 | −1.4 | −2.9 |
Male 30 dpt | |||||||||
Control | 10 | ||||||||
Anti-Gsdf-a | 2 | 1.2 | 2.5 | −1.2 | −1.6 | 1.2 | −1.9 | −1.8 | −2.1 |
Anti-Gsdf-b | 4 | 1.0 | 1.9 | −1.3 | −1.7 | −1.2 | −1.7 | −1.9 | −1.3 |
Anti-Gdf9-a | 3 | 1.0 | 1.5 | −1.2 | −1.6 | −1.4 | −2.5 | −1.7 | −1.4 |
Anti-Gdf9-b | 4 | 1.3 | 1.1 | 1.0 | −2.0 | −1.6 | −2.8 | −3.1 | 1.1 |
Anti-Cd205 | 5 | −1.2 | 2.2 | 1.2 | −2.2 | −1.8 | −3.2 | −3.1 | −2.2 |
The relative gene expression is shown as fold change compared to control fish. The values in bold represent a significant difference in expression (ANOVA and LSD test at p<0.05). Different antigens for the same target protein are indicated using –a or –b following the target protein name. All data were normalized using the reference genes rpl13α and ef1α.