Table 3. Expression of marker genes at 15 and 30 days post treatment (dpt).

Treatment	n	Gene
		tcrac	igu	bik	vasa	gsdf	inhα	amh	cyp19a1a
Female 15 dpt
Control	7
Anti-Gsdf-a	3	1.2	−1.4	−1.1	1.0	−2.0	1.2	1.3	1.7
Anti-Gsdf-b	3	−1.5	2.1	1.3	−1.6	−1.4	1.0	−1.1	−1.6
Anti-Gdf9-a	2	1.0	1.7	1.4	−1.6	−1.6	−1.1	1.0	1.4
Anti-Gdf9-b	6	−1.9	2.6	1.8	−2.1	1.5	−1.3	−1.1	−1.2
Anti-Cd205	5	−1.6	2.9	1.7	−1.5	1.0	1.0	1.2	−1.3
Male 15 dpt
Control	6
Anti-Gsdf-a	3	−1.9	−1.6	−1.3	1.4	1.2	1.7	1.7	−1.3
Anti-Gsdf-b	3	−2.6	2.5	−1.3	1.1	−1.1	1.5	1.0	−2.9
Anti-Gdf9-a	3	−3.0	−1.4	1.0	1.7	1.0	1.5	1.5	−3.3
Anti-Gdf9-b	3	1.2	1.2	1.4	−1.5	−1.3	−1.4	−1.1	−1.3
Anti-Cd205	4	−2.1	1.0	−1.3	−2.0	−1.3	−1.4	−1.2	−2.2
Female 30 dpt
Control	9
Anti-Gsdf-a	4	1.7	1.3	−1.3	1.7	−1.5	1.9	1.2	1.2
Anti-Gsdf-b	4	1.1	1.2	−1.7	1.2	−1.2	−1.3	1.0	−2.7
Anti-Gdf9-a	4	−1.9	2.4	1.2	−1.2	−1.3	1.1	−1.2	1.3
Anti-Gdf9-b	6	−1.4	2.2	−1.2	−1.3	1.5	−1.8	−1.7	−2.3
Anti-Cd205	6	−2.1	3.7	1.4	−1.4	1.3	−1.6	−1.4	−2.9
Male 30 dpt
Control	10
Anti-Gsdf-a	2	1.2	2.5	−1.2	−1.6	1.2	−1.9	−1.8	−2.1
Anti-Gsdf-b	4	1.0	1.9	−1.3	−1.7	−1.2	−1.7	−1.9	−1.3
Anti-Gdf9-a	3	1.0	1.5	−1.2	−1.6	−1.4	−2.5	−1.7	−1.4
Anti-Gdf9-b	4	1.3	1.1	1.0	−2.0	−1.6	−2.8	−3.1	1.1
Anti-Cd205	5	−1.2	2.2	1.2	−2.2	−1.8	−3.2	−3.1	−2.2

The relative gene expression is shown as fold change compared to control fish. The values in bold represent a significant difference in expression (ANOVA and LSD test at p<0.05). Different antigens for the same target protein are indicated using –a or –b following the target protein name. All data were normalized using the reference genes rpl13α and ef1α.