. 2014 Dec 1;9(12):e114209. doi: 10.1371/journal.pone.0114209

Table 6. PCR primers used for quantitative real-time PCR with primer efficiency (E%), co-efficient of determination (R²), and amplicon size values.

Gene	Accession ID:	Primer sequence	E%	R²	Size
ß-actin*	ENSDART00000055194	Fw: CGAGCTGTCTTCCCATCCA	94.1	0.999	84
		Rv: TCACCAACGTAGCTGTCTTTCTG
ef1α*	ENSDART00000023156	Fw: CTGGAGGCCAGCTCAAACAT	93.9	0.999	85
		Rv:ATCAAGAAGAGTAGTACCGCTAGCATTAC
rpl13α*	NM_212784	Fw: TCTGGAGGACTGTAAGAGGTATGC	91.9	0.999	148
		Rv: AGACGCACAATCTTGAGAGCAG
tcrac	AF246178	Fw: CACAACGAGTTCAACATTACCGA	89.8	0.999	194
		Rv: CCAGAAGATGCCCAGTGACAA
igμ	AF281480	Fw: AGCGGAATGATAGCAGGGAGA	89.0	0.999	235
		Rv: TTGGTGAAATGGAATTGTGGA
bik	NM_001045038	Fw: AGATTATGCCGATATTCAGGACG	86.5	0.999	213
		Rv: CCCTCTGTCACATTCAGCCTTC
vasa	NM_131057	Fw: TCAGAGCAACAGGTAATGAGC	90.5	0.998	195
		Rv: CTACAGATGTGGCGACCAGAAC
gsdf	NM_001114668	Fw: GAACGCTCCTGAATCCACAGAC	90.5	0.999	210
		Rv: AATGACTCCCGCAGATGCTC
inhα**	NM_001045204	Fw: AGCCTCCTCTGCCAGTGTTG	97.7	0.996	188
		Rv: AGCATCAGAAGAGTGGTCAGGTA
amh	AY721604	Fw: TGCTCCTGTTCAGTGTCAATCC	77.5	0.992	180
		Rv: ATGTCTCAACCATCGTCTTCAGT
cyp19a1a	BC163008	Fw: AGATGTCGAGTTAAAGATCCTGCA	93.9	0.999	131
***		Rv: CGACCGGGTGAAAACGTAGA

Primers previously validated by Tang et al., 2007 [63] (*), Poon et al., 2009 [47] (**), and Jørgensen et al., 2008 [62] (***).

Table 6. PCR primers used for quantitative real-time PCR with primer efficiency (E%), co-efficient of determination (R2), and amplicon size values.