Figure 5.

Expression of neurotransmitter-activated channels in human induced pluripotent stem cell (hiPSC) differentiated neurons. (A): Representative examples of ionic currents induced by application of 1 mM GABA, 1 mM glycine, or 100 μM ACh in differentiated hiPSCs. Whole-cell recordings with intracellular solution containing 20 mM Cl⁻ at holding potential 0 mV (for GABA and glycine) and at −40 mV for ACh. Bars above traces indicate the time of agonist application. (B): Current-voltage relationship of the GABA-evoked inward current. Example (top) of superimposed traces of GABA-evoked currents at different potentials. Reversal potential was approximately −50 mV, as predicted from Nernst equation for intracellular solution containing 20 mM KCl. (C): Dose-response dependency and superimposed traces (insert) of whole-cell currents induced by rapid application of different concentration of GABA. Recording at 0 mV. Abbreviations: ACh, acetylcholine; EC₅₀, half maximal effective concentration; s, seconds.