Cell-seeding efficiency of β-tricalcium phosphate (β-TCP). (A): The overall cell-seeding efficiency is shown at different time intervals (15, 30, and 60 minutes) following seeding of the scaffold with cells. (B): The cell-seeding efficiency at the different time intervals was stratified by the temperature at which the cells were allowed to incubate with the scaffold. (C): SEM images at low and high magnification showing the distribution of cells and cell attachment to one β-TCP particle (individual particle sizes ranged from 500 to 1,000 μm). ∗, p < .05 relative to the 15-minute condition. Abbreviation: RT, room temperature.