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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: J Mol Cell Cardiol. 2014 Aug 14;0:26–32. doi: 10.1016/j.yjmcc.2014.08.007

Fig. 6.

Fig. 6

Effect of antisense MO on Kv11.1 isoform expression in in canonical poly(A) signal construct. (A) RPA analysis of mRNA from cells stably expressing the short KCNH2 gene containing the canonical poly(A) signal following the treatment with 10 μM invert or antisense MO. Signals were quantified and shown as an isoform percentage of the total signal (1a+1a-USO, n=3). (B) Immunoblot analysis showing concentration dependence of antisense MO effect. Signals were quantified and shown as an isoform percentage of total (1a+1a-USO) Kv11.1 protein (mean±SD, n=3). (C) Representative currents recorded from Flp-In HEK293 cells stably expressing short KCNH2 gene containing the canonical poly(A) signal following treatment with 10 μM invert or antisense MO for 48 h.