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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Int Immunopharmacol. 2014 Oct 12;23(2):516–522. doi: 10.1016/j.intimp.2014.09.027

Figure 2. Suppressive ODN promote the generation of human iTreg in vitro.

Figure 2

CD4+CD25− T cells were isolated from normal healthy volunteers (N=3) and incubated for 2 h with 3 uM of suppressive ODN in medium supplemented with IL-2, anti-CD3/CD28 and TGFβ1 as described in the methods section. The cells were stained to detect expression of CD4 and FoxP3 and analyzed by FACS on day 5. Each donor was analyzed independently on a different day and results show the mean + SD of all samples.

*, p<0.05; **, p<0.01