Figure 4.

Actin stabilization disrupts GnRH-dependent Ca²⁺ sparklet stimulation. A, Representative traces showing time courses of Ca²⁺ influx in αT3–1 cells exposed to GnRH (3 nM) in the presence or absence of jasplakinolide (100 nM for 5 min). B and C, Plots of Ca²⁺ sparklet site activities (nP_s) and mean ± SEM Ca²⁺ sparklet site densities (Ca²⁺ sparklet sites per square micrometer) for GnRH (3 nM; n = 9 cells in each group) with or without jasplakinolide (100 nM for 5 min). D, Western blot analysis of ERK activation (as measured by ERK phosphorylation) in αT3–1 cells exposed to GnRH (3 nM) with or without jasplakinolide (100 nM for 5 min; n ≥ 3 independent experiments). *, P < .05; †, P < .05 vs jasplakinolide and P < .05 vs GnRH. cont, control; jas, jasplakinolide.