Figure 5.

Western blot analysis of multiple intracellular molecules in RIP-HGF TG mouse islets with IRS2 deficiency that are involved in β-cell proliferation and survival. A–C, Analysis of AKT Ser473 phosphorylation (A), FoxO1 Ser256 phosphorylation (B), and Pdx-1 levels (C) in islet extracts from WT, TG, KO, and TG/KO mice (n = 4/group). A representative Western blot is shown at the top of the graph with the calculation of densitometry of 4 different blots. Actin was used as a housekeeping protein. Results are means ± SEM. *, P < .05 vs the other 3 groups of mice; ∧, P < .05 vs WT; #, P < .05 vs WT and KO mice. D, Representative photomicrographs of pancreatic sections from WT, TG, KO, and TG/KO mice stained for Pdx-1 (red), insulin (green), and DAPI (blue). Inset: higher magnification image of specific islet areas stained for Pdx-1. Notice the Pdx-1 nuclear staining in WT, TG, and TG/KO mice and the more diffused cytoplasmic staining in KO mice.