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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Microb Pathog. 2014 Sep 16;0:33–43. doi: 10.1016/j.micpath.2014.08.010

FIG. 3.

FIG. 3

Results of semi-quantitative RT-PCR of the aerobic glycerol metabolic pathway. Y. pestis KIM5 were pre-grown with aeration in BCS medium supplemented with 0.2% K-gluconate at 26°C for 24 hours with two transfers. At OD600 = 0.3 (0 hours time point) additional carbon sources were added and temperature from 26°C to 37°C was changed in half of the samples. Approximately 1×108 bacteria were taken at 1 hour and 4 hours post-temperature shift from both 26°C and 37°C cultures grown in the presence of either 0.2% K-gluconate alone (K); both 0.2% K-gluconate and 0.2% glycerol (KY); or 0.2% K-gluconate, 0.2% glycerol, and 0.2% glucose (KYU). RT-PCR conditions consisted of 25 ng total RNA for 25 cycles of amplification. Expression values were derived from ImageJ software analyses and normalized to gyrB. Color scale reflects relative percentile changes in gene expression from the mean.