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. Author manuscript; available in PMC: 2015 Oct 10.
Published in final edited form as: Biochem Biophys Res Commun. 2014 Sep 27;453(1):185–191. doi: 10.1016/j.bbrc.2014.09.085

Figure 4.

Figure 4

Regulation of titin translational efficiency. A: NRVM were transiently transfected with 100ng RTK and 200ng pGL3 Control or Ttn 5′ UTR via lipofectamine delivery for 24 hours. NRVM were either treated with vehicle, 0.5μM doxorubicin or 50μM H2O2 for 24hrs. Cells were lysed and assayed for luciferase activities (n=3). B: NRVM were transiently transfected with 100ng RTK and 200ng Ttn 5′-UTR or mutated rs13422986 5′-UTR for 24hrs. Cells were lysed and assayed for luciferase activities (n=4). All data presented as Mean±SEM, *p<0.05 vs 5′-UTR. Groups were analyzed by ANOVA or student’s t-test.