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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Nat Cell Biol. 2014 Nov 24;16(12):1192–1201. doi: 10.1038/ncb3063

Figure 5. Effects of processivity-enhancing fragment CC1B on dynein stepping behavior.

Figure 5

Sample dynein bead traces with detected steps (in red) for single dynein alone (A) and with CC1B (B). Arrows indicate points of force feedback (see Methods). (C) Distribution of step-sizes for single dyneins with (blue) or without (red) CC1B (428 stepping events). CC1B reduces back-stepping (green arrow) and increases large forward steps (blue arrow). (D) Sample traces showing lateral bead position on microtubule vs. time for stationary dynein (without ATP - top); and dynein alone (middle) or with CC1B (bottom) moving on a microtubule in the presence of saturating ATP. Step detection identifies changes in lateral bead position (red lines); δ indicates size of detected lateral step in nm. (E) Lateral dynein bead switching frequency (95 switching events) on the microtubule surface, and (F) as function of run length (94 run lengths); p value from t-test. Longer CC1B-dynein runs correlate with decreased lateral switching frequency. (G) Magnitude of lateral step size (428 detected steps). CC1B decreases the frequency and magnitude of lateral dynein steps. Error bars are SEM.