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. Author manuscript; available in PMC: 2014 Dec 2.
Published in final edited form as: Exp Hematol. 2014 Jul 14;42(10):857–861.e1. doi: 10.1016/j.exphem.2014.06.007

Figure 1. C1 Inhibition blocks APC-mediated Hemolysis in PNH Erythrocytes.

Figure 1

A. The erythrocytes of patient 1 were incubated for 1 hour at 37° C with acidified (pH 6.4) normal human serum(aNHS) (1:3) and acidified heat-inactivated serum (aNHS[H])(1:3) containing increasing concentrations of C1INH (0-12 Units/mL). Hemolysis was measured using the concentration of supernatant hemoglobin (determined by spectrophotometry at 415 nm).

B. The erythrocytes of patients 2-5 (treated with eculizumab) were incubated as above with increasing concentrations of C1INH (0-6 Units/mL). Hemolysis was measured again by spectrophotometry at 415 nm.

C. The erythrocytes of patient 6 (not treated with eculizumab) were incubated as above at increasing concentrations of C1INH (0-12 Units/mL) and hemolysis measured.