Fig 2.

Percentage of non-apoptotic spermatozoa in neat semen sample, sperm sample prepared before freezing (SBF), and sperm sample prepared after freezing-thawing (SAFT) in 65 semen samples evaluated by Flow cytometric analysis. a. The percentage of non-apoptotic spermatozoa was not significantly difference between NS and SBF, but the percentage of non-apoptotic spermatozoa showed significantly decreased (P <0.001) in SAFT compared with NS. b. The percentage of non-apoptotic sperm before and after cryopreservation in each sample in SBF group. c. The percentage of non-apoptotic sperm before and after cryopreservation in each sample in SAFT group