Table 3.
Distribution of non-apoptotic sperm, apoptotic sperm, and necrotic sperm in neat semen samples and semen samples prepared by swim-up before and after cryopreservation in 65 semen samples, evaluated by flow cytometric analysis
| Neat semen sample (NS,%Rate) | Semen samples prepared before freezing group (SBF,% Rate) | Semen samples prepared after freezing- thawing group (SAFT,%Rate) | P value | ||||
|---|---|---|---|---|---|---|---|
| Mean | SD | Mean | SD | Mean | SD | ||
| Non-apoptotic sperm a,c | 65.2 | 14.0 | 64.5 | 18.0 | 38.4 | 18.6 | < 0.001 |
| Early apoptotic spermb,c | 3.8 | 2.9 | 1.5 | 0.8 | 3.1 | 2.0 | < 0.001 |
| Late apoptotic sperma,b,c | 20.6 | 9.4 | 30.8 | 16.6 | 48.5 | 16.3 | < 0.001 |
| Total apoptotic sperma,c | 24.4 | 11.1 | 32.3 | 16.9 | 51.6 | 16.6 | < 0.001 |
| Necrotic spermb,c | 10.4 | 6.2 | 3.2 | 3.0 | 10.0 | 6.5 | < 0.001 |
Hoc analysis using Bonferroni multiple-comparisons test (P < 0.05)
a Significance of difference between NS and SAFT
b Significance of difference between NS and SBF
c Significance of difference between SAFT and SBF