Table 2.
Effect of different concentrations of auxin or cytokinin alone or in combination on percent callus formation, callus color, callus texture and callus growth in Stevia rebaudiana (Bert.).
| Plant growth regulators (mg l−1) | Callus formation (%) | Callus color | Callus texture | Callus growth |
|---|---|---|---|---|
| 2,4-D (0.5) | 66.48 ± 0.90cd⁎ | Yellowish green | Granular and spongy | +++ |
| 2,4-D (1.0) | 66.54 ± 1.62cd | Yellowish green | Granular and spongy | +++ |
| 2,4-D (1.5) | 66.94 ± 1.75c | Yellowish green | Granular and spongy | +++ |
| 2,4-D (2.0) | 83.23 ± 2.67a | Yellowish green | Granular | +++ |
| 2,4-D (3.0) | 50.37 ± 3.01e | Yellowish green | Granular | +++ |
| 2,4-D (4.0) | 83.25 ± 2.10a | Yellowish green | Granular | ++++ |
| BA (1.0) | 29.33±4.35g | Green | Compact | + |
| BA (2.0) | 70.06 ± 2.73b | Green | Compact | +++ |
| BA (3.0) | 40.38 ± 2.89f | Green | Compact | ++ |
| 2,4-D + BA (0.5) | 66.28 ± 1.25cd | Yellowish green | Granular | +++++ |
| 2,4-D+BA (1.0) | 83.22 ± 2.02a | Yellowish green | Granular | +++++ |
| 2,4-D + BA (2.0) | 84.60±1.27a | Yellowish green | Granular | +++++ |
| 2,4-D+BA (3.0) | 64.23 ± 3.21d | Yellowish green | Granular | +++++ |
| Control | 0 | NA | NA | NA |
+++++ Excellent, ++++ Very good, +++Good, ++Poor, + Very poor.
⁎
Indirect regeneration of S. rebaudiana via callus cultures from leaf explants. Data was taken from replicated experiments. Mean data in each column with common letters and standard deviation are not significantly different at P < 0.05.