Table 3.
Shoot and root organogenesis in Stevia rebaudiana (Bert.) on half and full MS or B5 medium supplemented with different concentrations of cytokinin (BA, Zeatin), gibberellic acid (GA3) and auxin alone (IBA, NAA, 2,4-D) or in combination (BA+ GA3; BA+IBA; BA+ GA3 + IBA + NAA; BA + zeatin; IBA + NAA).
| Plant growth regulators (mg l−1) | % Shooting mean ± SD | No. of shoots per explant | Mean shoot length (cm) | % Rooting mean ± SD | Mean root length (cm) | No. of roots per plantlet |
|---|---|---|---|---|---|---|
| BA 1.0 | 85.00 ± 0.67d⁎ | 20 ± 1.0e | 5.2 ± 2e | 53.13 ± 0.14e | 9.0 ± 0.5bc | 7.5 ± 0.37c |
| BA 2.0 | 96.00 ± 1.33a | 96 ± 4.8b | 6.3 ± 2e | 77.27 ± 0.24b | 4.5 ± 0.5 cd | 4 ± 0.2d |
| BA 3.0 | 70.00 ± 0.93 g | 106 ± 5.3a | 23.5 ± 0.5b | 46.14 ± 0.53f | 8.3 ± 0.6bc | 8.44 ± 0.5c |
| BA 4.0 | 60.00 ± 0.89jk | 37.5 ± 1.87d | 29.5 ± 0.59a | 46.43 ± 0.42f | 10.6 ± 1.5b | 13.5 ± 0.6b |
| BA 1.5 + GA3 0.5 | 90.00 ± 1.33b | 17 ± 0.85ef | 9.5 ± 0.5d | – | ||
| BA 1.0 + GA3 1.0 | 65.00 ± 0.67ij | 12 ± 0.6 fg | 11 ± 1.0 cd | – | ||
| BA 0.2 + GA3 0.2 | 66.60 ± 0.16hi | 45 ± 2.25c | 5.75 ± 0.7e | – | ||
| BA 1.0 + 0.1(GA3 + IBA + NAA) | 72.00 ± 1.33 fg | 44 ± 2.2c | 12 ± 1.0c | – | ||
| BA 1.0 + IBA 0.2 + 0.3(GA3 + NAA) | 86.66 ± 0.96 cd | 37.5 ± 1.8d | 5.1 ± 0.65e | – | ||
| BA + GA3 (0.2) + NAA + IBA (0.5) | 73.68 ± 0.75f | 12 ± 0.6 fg | 13.2 ± 1.0c | – | ||
| ½ MS + 0.2(BA + GA3) + 0.5(NAA + IBA) | 87.5 ± 0.96c | 23 ± 1.15e | 10 ± 1.0 cd | – | ||
| BA 0.5 + IBA 1.0 | 66.66 ± 0.16hi | 32.2 ± 1.6de | 11.5 ± 1.47 cd | – | ||
| BA 0.5 + ½ MS IBA | 80.00 ± 1.33e | 4.0 ± 0.2 g | 3.1 ± 1.0f | – | ||
| BA 1.0 + Zeatin 0.1 | 67.00 ± 0.67 h | 2.0 ± 0.1 g | 9.0 ± 0.5d | – | ||
| BA 1.0 + 0.5 (NAA + 2,4-D) | 20.00 ± 1.00 l | 2.5 ± 0.12 g | 3.0 ± 0.5f | – | ||
| IBA 1.0 | 64.28 ± 0.51j | 4.5 ± 0.25 g | 12 ± 0.5c | – | ||
| IBA 1.0 + NAA 0.5 | 50.00 ± 0.33 k | 2.5 ± 0.12 g | 5.5 ± 0.5e | – | ||
| IBA 0.5 + ½ MS NAA | 71.42 ± 0.41 g | 4.7 ± 0.17 g | 3.2 ± 0.19f | – | ||
| NAA 0.5 + IBA 0.5 | 71.42 ± 0.41 g | 2.3 ± 0.36 g | 3.7 ± 0.51f | 73.92 ± 0.41c | 7.4 ± 3.21c | 13.6 ± 0.8b |
| ½ MS + NAA 0.5 + IBA 0.5 | – | 85.72 ± 0.21a | 14.3 ± 1.0a | 19 ± 0.95a | ||
| BA 1.0 + B5 | – | 15.38 ± 0.38 g | 2.7 ± 2.0d | 4.5 ± 0.25d | ||
| BA 2.0 + B5 | – | 15.79 ± 0.21 g | 8.25 ± 0.5bc | 12 ± 0.6bc | ||
| ½ B5 + 0.5(IBA + NAA) | – | 70.00 ± 0.50d | 3.75 ± 3.0d | 7 ± 0.35c | ||
| Control | 8.00 ± 0.34 m | 1 ± 0.05 g | 5.5 ± 0.2e | 0 | 0 | 0 |
⁎
Data was taken from replicated experiments. Mean data in each column with common letters and standard deviation are not significantly different at P < 0.05.