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Steps for measuring the activity of the oxidoreductase NADH-cell pigment C.
| Reagent (mL) |
No saponin added (-S) |
Saponin added (+S) |
||
| Blank | Sample | Blank | Sample | |
| 0.6 mg protein/mL Membrane suspension | 0.6 | 0.6 | 0.6 | 0.6 |
| PBS (5p8.4) | 2.4 | 2.2 | 2.2 | 2 |
| 0.1% saponin | / | / | 0.2 | 0.2 |
| 5 mmol/L K3Fe(CN)6 | / | 0.1 | / | 0.1 |
| Let the solution stand for 10 min, added NADH, and recorded the result after 1 min 6 mmol/L NADH | / | 0.1 | / | 0.1 |
