Figure 3.

Sketch map of structure of plasmid pUC-HBV1.2 and the mutation sites of all constructed plasmids. Panel A: Upper line and box figure indicate the 1.2 copy HBV genome cloned into pUC18 EcoRI and Hind III sites. Different open reading frames are indicated with corresponding box. Lower letters indicate HBV BCP and precore region sequence, TA rich region and DR region are underlined. Scheduled nucleosides to be mutated are indicated with numeral; Panel B: Recombinant constructs and their corresponding mutation sites. The constructs are listed at left, long gray box indicate consensus sequences, mutation nucleosides and their sites are indicated.