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. 2014 Dec;63(Pt 12):1644–1648. doi: 10.1099/jmm.0.080721-0

Fig. 1.

Fig. 1.

The 26.5 kb fragment harbouring the KPC gene in A. baumannii. The 26.5 kb DNA fragment (blue), which includes the 18.9 kb KQ-like element (bright green) and a 7.6 kb DNA fragment (brown) from Y. pestis PY-95 was inserted in the chromosome of A. baumannii. The KQ-like element consists of the truncated transposon Tn1331 backbone (light blue) interrupted by Tn5387 (light yellow) and Tn4401 (sea green). This 26.5 kb DNA fragment, flanked by ISEcp1 IRL and IRR-like sequences (black triangles), was inserted into an oxidoreductase gene (yellow arrow) generating a 7 bp duplication at the target sequence (vertical red line). Tn1331 was inserted in a truncated Y. pestis hypothetical protein (not shown) generating a 5 bp duplication at the target site (vertical red line) linking the KQ-like element with the 7.6 kb DNA fragment. Chromosomal proteins (plum arrows) flanking the 5′ and 3′ DNA region of the interrupted oxidoreductase gene were identified in the remaining sequence of contig 36. SnapGene 2.4.2 was used for map construction. ABC, ATP-binding cassette.