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. Author manuscript; available in PMC: 2014 Dec 15.
Published in final edited form as: Cell Rep. 2014 Oct 23;9(3):1007–1022. doi: 10.1016/j.celrep.2014.09.049

Figure 3. Developmental and Adult adipose progenitors have distinct lineages and localities.

Figure 3

A-C) AdipoTrak-GFP; SMA-RFP mice were administered TM at P10 or P30 and chased to P60: (A) sectioned SQ depots were analyzed for RFP and immunostained for PECAM (green) and Perilipin (blue), or (B) RFP and LipidTox (lipid) staining. C) Floated adipocytes were examined for RFP (mural lineage) and GFP (adipose lineage). Yellow arrows indicate co-localization between AdipoTrak-GFP and SMA-RFP labeled adipocytes. D-E) One day after a P10 or P30 TM pulse, AdipoTrak-GFP; SMA-RFP SV cells were isolated, cultured in adipogenic conditions, and Oil Red O (adipocyte) stained (D) or examined for RFP fluorescence (E). Yellow arrows (E) indicate SMA-RFP labeled adipocytes. F) Two days after a P10 or P30 TM pulse, AdipoTrak-GFP; SMA-RFP depots were examined for RFP and GFP expression and immunostained for SMA. Yellow arrows indicate AdipoTrak-GFP; SMA-RFP labeled progenitors in a perivascular position at P30. G-H) FACS analyses (G) and mRNA analyses (H) of denoted genes in FACS isolated RFP+ or RFP− cells from SMA-RFP mice two days post TM injection. *P<0.01 RFP+ versus RFP-− cells; Scale = 100 μm.