TABLE 4.
Expression of Genes Associated with Fibrosis
| Genes | AlbCre+/caNrf2− |
AlbCre+/caNrf2+ |
Dietary Effect |
Genetic Effect |
||||
|---|---|---|---|---|---|---|---|---|
| Std Chow | MCD | Std Chow | MCD | AlbCre+/caNrf2- | AlbCre+/caNrf2+ | Std Chow | MCD | |
| Columns | 1 | 2 | 3 | 4 | 1 versus 2 | 3 versus 4 | 1 versus 3 | 2 versus 4 |
| COL1A1 | 1.00 ± 0.11 | 0.80 ± 0.11 | 1.45 ± 0.29 | 1.13 ± 0.22 | — | — | — | — |
| TIMP1 | 1.00 ± 0.09 | 5.25 ± 0.82* | 1.75 ± 0.50 | 3.74 ± 0.53* | 5.25 | 2.14 | — | — |
| TIMP2 | 1.00 ± 0.08 | 1.05 ± 0.12 | 1.12 ± 0.13 | 1.03 ± 0.09 | — | — | — | — |
| MMP2 | 1.00 ± 0.14 | 0.99 ± 0.11 | 1.39 ± 0.10 | 1.26 ± 0.20 | — | — | — | — |
| MMP9 | 1.00 ± 0.09 | 0.91 ± 0.09 | 1.27 ± 0.28 | 0.96 ± 0.11 | — | — | — | — |
qRT-PCR was performed for genes associated with fibrosis using RNAs from AlbCre+/caNrf2− versus AlbCre+/caNrf2+ animals on MCD diet after 28 days. The mRNA levels are first normalized to the β-actin mRNA content of each sample, and each experimental group is then normalized to the AlbCre+/caNrf2−, standard chow group. All data are presented as mean ± SEM. N = 6–8 for each of the 4 groups. The fold changes in mRNA abundance were compared between groups using one-way ANOVA with Fisher’s LSD post hoc test. Significant fold changes are presented on the right side of the table. *P < 0.05 when compared with standard chow of the same genotype. **P < 0.05 when compared with AlbCre+/caNrf2− on the same diet.