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. 2014 Dec 2;4:7190. doi: 10.1038/srep07190

Figure 2. SUMOylation is required for long-term potentiation.

Figure 2

(a) LTP in area CA1 of hippocampal slices induced by theta burst stimulation of Schaffer collaterals. Treatments were perfused for 1 hour prior to LTP induction. Vehicle n = 14, TAT-Ubc9 n = 9, TAT-Ubc9(DN) n = 12, TAT-GFP n = 7. (b) Quantification of the last 10 minutes of LTP recordings. ANOVA of the averages, p = 0.0004; *TAT-Ubc9(DN) p < 0.05 versus all other groups. (c) Basal synaptic transmission, as measured by input-output curves, was not affected by TAT-Ubc9 or TAT-Ubc9(DN). (d) Recombinant GST-SENP1 has de-SUMOylating activity. In vitro SUMO2 conjugation assay using E2-25K substrate; representative avidin-HRP blots. The higher molecular weight band is SUMO2 conjugated substrate. Lane 1: positive control, lane 2: no ATP negative control, lane 3: reaction with GST-SENP1(C603S) inactive mutant, lane 4: reaction with GST-SENP1. (e) Whole cell patch clamp recordings of single-cell LTP in area CA1 induced by theta burst stimulation. Either vehicle, SENP1 or SENP1(C603S) inactive mutant was introduced intracellularly via the patch pipette. Vehicle n = 7, SENP1(C603S) n = 4, SENP1 n = 7. ANOVA of the post-tetanic time points p < 0.0001. (f) Paired-pulse facilitation (PPF) in area CA1 of hippocampal slices. Vehicle and TAT-Ubc9(DN) n = 6 each. Data presented as means ± SEM. See also Supplementary Figure S2.