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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Cryogenics (Guildf). 2014 July-August;62:118–128. doi: 10.1016/j.cryogenics.2014.04.017

Figure 10.

Figure 10

Combined adverse effects to cryopreservation captured in a single protocol on 6M DMSO: (a) homogeneous crystallization in a vitrified domain at the onset of fracturing (fractures are pointed by arrows) at the early stage of rewarming, (b) a dense fractures network 1.1 s after the pervious image, (c) disappearance of fractures due to either melting of the fractured areas or molten CPA flowing into the voids between fracture walls, (d) RPC correlating with the previously observed fractures at the same location, (e) opacity due to RPC, and (f) the corresponding thermal history in the sample.