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. 2014 Dec 1;3(12):751–761. doi: 10.1089/wound.2012.0418

Figure 4.

Figure 4.

Intrinsic pathway markers. Real time RT-PCR of (a) Trp53, (b) Casp2, (c) Casp9, (d) Cycs, and (e) Apaf1 were performed on RNA isolated from uninjured tissue (U) and wound samples at 6 h, 24 h, D3, D5, and D7 postinjury. To determine relative changes in mRNA levels during wound healing, all samples were normalized to Gapdh expression in uninjured tongue tissue. The results are shown as the mean±SEM; n=3. Data were analyzed by two-way ANOVA and Bonferroni's posttest (*p<0.05 for skin vs. tongue wounds, # p<0.05 for skin vs. uninjured skin, &p<0.05 for tongue vs. uninjured tongue).