FIG. 3.

Immune reactivity following adoptive transfer of splenocytes from previously treated immunocompetent animals. Splenocytes were harvested from each of the animals depicted in Fig. 2a–d and infused into naïve C57BL/6 mice. One day later, the recipient mice were hydrodynamically injected with 5 μg pT2/CaL plus 0.5 μg pCMV-SB100X. Each subfigure is titled according to the source of splenocytes used for each of the injections, that is, the original C57BL/6 treatment groups described in Fig. 1 and expression detailed for individual mice in Fig. 2. (a) C57BL/6 co-injected with pT2/CaL plus pCMV-SB100X (Fig. 2a). (b) C57BL/6 injected with pT2/CaL alone (Fig. 2b). (c) CP immunosuppressed C57BL/6 co-injected with pT2/CaL plus pCMV-SB100X (Fig. 2c). (d) CP immunosuppressed C57BL/6 injected with pT2/CaL alone (Fig. 2d). Each line represents an individual mouse. Luciferase expression (log₁₀ of p/sec/cm²) is plotted over a period of 20 weeks.