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. 2014 Feb 18;63(12):1854–1863. doi: 10.1136/gutjnl-2013-306508

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A well-orientated Barrett's gland. (A) An H&E; (B and C) stained with MUC5AC and MUC2 (figure 3B prelaser capture microdissection (LCM), figure 3C post-LCM). (D) Cells microdissected from the gland all contain the same heteroplasmic m.825 G>T mutation in the MT-RNR1 gene. MUC2 cells (i) wild-type cells; (ii) MUC2 cells, (iii) MUC5AC cells, (iv) basal mucous-secreting cells (note: a lower level of heteroplasmy was detected), (iv) all share this mutation, but cells from the neighbouring wild-type gland do not. Online supplementary figure S4 shows high power views of the cells dissected.