Table 1.

Thermal transition temperatures of ASWT and mutants.

Mutant	TM50	TM1	TM2	TM3	ΔH1	ΔH2	ΔH3	ε1	ε2	ε3	n
	Degrees celsius				Kcal/Mol
ASWT	45.3 ± 0.3	28.1 ± 2.5	n/a	45.5 ± 0.1	−22 ± 8	n/a	−144 ± 29	0.38 ± 0.03	n/a	0.64 ± 0.04	9
E40K	44.0 ± 0.2*	38.2 ± 0.8‡	43.9 ± 0.2	51.8 ± 1.0‡	−38 ± 12	−146 ± 36	−128 ± 34	0.39 ± 0.08	0.41 ± 0.06	0.18 ± 0.05	9
E54K	45.3 ± 0.1	37.8 ± 0.6‡	44.3 ± 0.1	52.6 ± 0.3‡	−36 ± 10	−146 ± 23	−118 ± 22	0.39 ± 0.07	0.36 ± 0.06	0.24 ± 0.05	9
E62Q	47.9 ± 0.5‡	37.9 ± 0.4‡	45.1 ± 0.1	51.5 ± 1.0‡	−30 ± 7	−149 ± 64	−141 ± 48	0.35 ± 0.09	0.35 ± 0.11	0.29 ± 0.19	11
L185R	46.8 ± 0.3*	30.4 ± 1.9	n/a	46.5 ± 0.1	−24 ± 6	n/a	−155 ± 33	0.39 ± 0.01	n/a	0.61 ± 0.02	9

Shown are the results of thermal denaturation spectra fitted to a two and three-transition model (see methods for description). The values represent mean ± SE of the values acquired from the fits. The melting temperature (T_M50), transition temperatures (T_{M1, 2, 3}), corresponding enthalpy values (ΔH) and extinction coefficients (ε) for each mutant are reported for the spectra measured the number of times shown (n). Statistical significance for temperatures were determined by ANOVA followed by Dunnett's multiple comparisons test;

^*p < 0.05,

^‡p < 0.001.