Figure 4.

Cx43 and Panx1 unopposed channel activity evoked by prenatal nicotine and postnatal HFC diet depended on the iNOS/COX₂/EP₁ receptor pathway and NMDA/P2X₇ receptor signaling. Averaged data normalized to maximal Etd uptake ratio (dashed line) achieved by prenatal nicotine and postnatal HFC diet in microglia (white bars), astrocytes (gray bars) and neurons (black bars) treated with the following blockers during Etd uptake recordings: L-N6 (1 µM), ns-398 (5 µM), sc-19220 (20 µM), BBG (200 µM), oATP (10 µM) and CPP (20 µM). * p < 0.05, ** p < 0.005; *** p < 0.001, effect of each blocker compared with the effect induced by prenatal nicotine plus a postnatal HFC diet. Averaged data were obtained from at least three independent experiments.