Figure 5.

Opening of Cx43 and Panx1 unopposed channels allowed for the release of glutamate and ATP via the iNOS/COX₂/EP₁ receptor pathway and NMDA/P2X₇ receptor signaling. Averaged data of glutamate (A) or ATP (B) release by hippocampal slices made from mice maintained under control conditions (dashed bar), or exposed to prenatal nicotine (white bar), postnatal HFC diet (gray bars) or a combination of both (black bars). Also shown are the effect of the following blockers applied during the experiments: TAT-L2 (100 µM), Gap19 (100 µM), Gap26 (100 µM), ¹⁰panx1 (100 µM), probenecid (Prob, 500 uM), L-N6, ns-398, sc-19220, BBG, oATP or CPP. ** p < 0.005, effect of prenatal nicotine plus postnatal HFC diet compared with control conditions. ^# p < 0.05, ^## p < 0.005; effect of each blocker compared with the effect induced by prenatal nicotine plus a postnatal HFC diet. Averaged data were obtained from at least three independent experiments.