Table 5.
Cellular composition of peripheral lymph nodes and spleena
| Cells of interest | plt/plt/Ldlr−/− (n=9) ± SD | Control (n=9) ± SD | P-value |
|---|---|---|---|
| Peripheral lymph nodes | |||
| CD3+ | 28.78±5.07 | 31.60±2.74 | NS |
| CD3+/CD4+ | 12.64±1.52 | 15.62±1.68 | NS |
| CD3+/CD8+ | 6.10±3.89 | 7.64±5.50 | NS |
| CD3+/CD4+/CD8+ | 2.32±1.21 | 2.67±0.81 | NS |
| CD3+/CD4+/CD44+ | 5.29±1.18 | 9.15±2.57 | 0.0163 |
| CD19+ | 40.17±5.06 | 40.72±3.34 | NS |
| CD19+/CD25+ | 4.67±1.69 | 5.02±2.73 | NS |
| CD11c+ | 0.98±0.17 | 1.03±0.41 | NS |
| CD11c+/CD83+ | 0.21±0.07 | 0.22±0.09 | NS |
| Spleen | (n=5) ± SD | (n=5) ± SD | |
| CD3+ | 35.56±4.96 | 36.42±5.47 | NS |
| CD3+/CD4+ | 13.48±3.22 | 13.25±2.39 | NS |
| CD3+/CD8+ | 7.56±2.48 | 8.15±1.86 | NS |
| CD3+/CD4+/CD8+ | 4.21±0.87 | 4.16±0.54 | NS |
| CD3+/CD4+/CD44+ | 7.36±2.69 | 8.05±1.85 | NS |
| CD19+ | 44.23±7.15 | 46.89±6.22 | NS |
| CD19+/CD25+ | 6.72±2.43 | 5.75±2.96 | NS |
| CD11c+ | 1.64±0.31 | 1.25±0.38 | NS |
| CD11c+/CD83+ | 0.24±0.05 | 0.27±0.06 | NS |
Notes:
a
Flow-cytometry analysis was performed to analyze the amount of T-cells (CD3/CD4/CD8/CD44), B cells (CD19/CD25), dendritic cells (CD11c/CD83), their subtypes, and grade of activation in peripheral lymph nodes and spleen. Values are shown as percentage of positive cells/total leukocytes in lymph node or spleen and as means ± SD.
Abbreviations: SD, standard deviation; NS, not significant.