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. Author manuscript; available in PMC: 2014 Dec 2.
Published in final edited form as: Cell Metab. 2008 Feb;7(2):125–134. doi: 10.1016/j.cmet.2007.11.013

Figure 2. Insulin resistance alters gene expression.

Figure 2

(A) Expression of the PGC and SREBP expression was measured in the livers of two-month old, non-fasted mice on a chow diet by real time PCR analysis or immunoblotting of liver extracts (PGC-1) or nuclear extracts (SREBP-1). SREBP-1 protein was also measured in two-month old mice after fasting and re-feeding (n=4-8, *p<0.05,). (B) Real time PCR analysis of cDNA prepared from the livers of two to four month old non-fasted chow fed mice. See text for gene names. (C) Two-month old Lox and LIRKO mice were gavaged with 40 mg/kg LXR agonist (T090137) or vehicle every 24 hours for two doses, and sacrificed four hours after the second dose, in the non-fasted state. Real time PCR was performed on cDNA prepared from these livers (n=5-8, *p<0.05, **p<0.005). (D) Hepatocytes were isolated from two to three month old Lox and LIRKO mice, and cultured overnight in the presence of 100 nM insulin, and either 5 μM LXR agonist or vehicle. RNA was extracted and subjected to real time PCR analysis. (n=4-6, *p<0.05)