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. Author manuscript; available in PMC: 2014 Dec 2.
Published in final edited form as: J Clin Cell Immunol. 2014 Sep 30;5(5):257. doi: 10.4172/2155-9899.1000257

Figure 3.

Figure 3

Effect of MUC18 over-expression (OE) on IL-8 protein levels in cultured normal human primary tracheobronchial epithelial (NHTE) cells. Cells were transduced with MUC18 cDNA or green fluorescence protein (GFP) gene (control) encoded in replication-deficient lentivirus. After 72 hours of transduction, cells were stimulated with polyI:C (10 μg/ml) for 4 and 24 hours. (A) IFN-β mRNA fold changes in polyI:Cstimulated NHTE cells with or without MUC18 over-expression (MUC18 OE). MUC18 OE cells, as compared to non-OE cells, show less IFN-β mRNA induction (fold change over cells without polyI:C) following polyI:C treatment at 4, but not 24 hours. Data are expressed as means ± SEM. N=4 human donors. (B) When the IFN-β mNRA data are expressed as net changes (MUC18 over-expression (OE) minus no MUC18 OE), MUC18 OE also decreases the induction of IFN-β mNRA expression 4 hours after polyI:C stimulation in NHTE cells. N=4 human donors. The horizontal bars indicate means.