Figure 2.

Analysis of B cell development in Nek2 Tg mice. (a) Hardy fractions from bone marrow (fraction (A) to (F)) were gated as follows: (A) B220⁺CD43⁺BP-1⁻CD24⁻; (B) B220⁺CD43⁺BP-1⁻CD24⁺; (C) B220⁺CD43⁺BP-1⁺CD24⁺; (D) B220⁺CD43⁻IgM⁻IgD⁻; (E) B220⁺CD43⁻IgM⁻IgD⁺; and (F) B220⁺CD43⁻IgM⁺IgD⁺. Fractions (A) and (B)–(D) may also be gated as CD19⁻ and CD19⁺ populations among B220⁺IgM⁻ cells, respectively (b) IgM⁺ splenocytes were separated into the following subsets: (T1), CD93⁺CD23⁻; (T2), CD93⁺CD23⁺IgM^int⁡; marginal zone (MZ), CD93⁻CD23⁻IgM^hiCD21^hi; and follicular (Fo), CD93⁻CD23⁺. (c) Peritoneal lymphocytes were separated into the following subsets: (B-2), CD19⁺B220^hi; (B-1), CD19⁺B220^lo-int; (B-1a), CD5⁺CD43⁺; and (B-1b), CD5⁻CD43⁻.