Figure 4.

Nek2 is expressed in germinal centers and Nek2 transgene mice develop spontaneous germinal centers. (a) Frozen sections of Nek2 transgenic positive and negative mice were stained with PNA. Sequential images were taken under fluorescence microscope. Spontaneous germinal centers were indicated by arrow and high magnification (right panel). (b) Frequency of spontaneous germinal centers observed in Nek2 transgenic positive and negative mice. ((c)-(d)) C57BL6 mice (n = 3) were immunized with SRBC for 10 days. Frozen sections of spleen were stained with Nek2 (Red) and PNA (green). Fluorescence image was taken under 10x (a) and 40x (b). (e) RT-qPCR analysis of Nek2 mRNA expression in B220⁺PNA⁻ and B220⁺PNA⁺ cells sorted from splenocytes of SRBC immunized C57BL6 mice (n = 3).