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. 2014 Nov 15;14:444. doi: 10.1186/1472-6882-14-444

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© Wu et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effects of biochanin A on protein expression of MMP-1,-3, −13, and TIMP-1 in interleukin-1β (IL-1β)–induced chondrocytes. Chondrocytes were incubated for 24 hours with IL-1β or a combination of biochanin A and IL-1β. Protein levels of MMP-1, −3, −13, and TIMP-1 were determined by Western blotting. β-actin was used as a loading control in the Western blotting.