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Effects of biochanin A on IκB-α degradation and NF-κB activation in IL-1β -induced chondrocytes. Chondrocytes were pre-treated with various concentrations of biochanin A for 2 h, followed by co-incubation with IL-1β (10 ng/ml) for 24 h, and harvested for Western blotting analysis. Biochanin A suppressed IL-1β-induced degradation of IκB-α and then attenuated IL-1β -stimulated activation of NF-κ B.
