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. 2014 Nov 26;9:5461–5469. doi: 10.2147/IJN.S70777

Figure 4.

Figure 4

HPLC elution profiles of fluorescent AGE products in control solution and in the reaction mixtures containing GNPs after 21 days of incubation at 37°C.

Notes: All reaction mixtures except for the control solution contained BSA (35 mg/mL), D-ribose (20 mM), and GNPs of various total surface area. Control solution contained BSA (35 mg/mL) with D-ribose (20 mM) only. Repeat experiments confirmed the reproducibility of the HPLC elution profiles for each of the incubation mixtures with AGE products appearing each time at retention times of 2.25 and 15.87 minutes. Under equivalent elution conditions, the blank solution containing BSA alone yielded no fluorescence absorbing AGE peaks demonstrating that for glycation to occur both protein and sugar were required (data not shown).

Abbreviations: HPLC, high performance liquid chromatography; AGE, advanced glycation end products; BSA, bovine serum albumin; GNP, gold nanoparticles; TS, total surface area.