Table 1.

Constituents of colloidal suspension mixtures A and B and a comparison of UV intensity at 280 nm of samples incubated for 21 days

Incubation mixture	Particle diameter (nm)	Mass of gold/mL (g/mL)	Particle number/mL	Total particle surface area/mL (nm2/mL)	UV absorbance at 280 nm (AU)	% glycation
A	2 nm	1.21×10−5	1.5×1014	18.8×1014	0.180±0.008	46.9%
	5 nm†	6.32×10−5	5.0×1013	39.3×1014	0.151±0.005	39.3%
	20 nm	5.66×10−5	7.0×1011	8.79×1014	0.269±0.010	70.1%
B	2 nm	5.65×10−6	7.0×1013	8.79×1014	0.259±0.006	67.4%
	5 nm	1.26×10−5	1.0×1013	8.79×1014	0.263±0.006	68.5%
	20 nm	5.66×10−5	7.0×1011	8.79×1014	0.269±0.010	70.1%
Control solution	Not applicable	Not applicable	Not applicable	Not applicable	0.384±0.011	100%
BSA blank	Not applicable	Not applicable	Not applicable	Not applicable	0.004±0.000	1.04%

Notes:

^†This solution had the highest GNP total surface are; denotes that the highest total surface area occurred in mixtures of A that contained 5 nm diameter GNPs (r=2.5 nm) and colloidal concentrations (C) of particles/mL. The number 15.7×10¹⁵ nm²/mL was derived by using the formula 4πr²×C to represent GNPs’ total surface area. All colloidal suspensions labeled B had a total surface area of 3.52×10¹⁵ nm²/mL. Percent glycation was determined on the basis of the UV reading of each sample relative to that of the control solution set at 100%.

Abbreviations: UV, ultraviolet; BSA, bovine serum albumin; GNP, gold nanoparticles; r, radius.