Component | Volume (μL) | Final |
---|---|---|
siLuc siRNA guide strand (100 μM) | 2 | 200 pmol |
[γ]-32P-ATP | 6 | 1 mCi |
SUPERase-In (40 U/μL) | 1 | 1.33 U/μL |
10x T4 PNK Buffer | 3 | 1x |
T4 PNK enzyme (10U/μL) | 3 | 1 U/μL |
Nuclease-free water | 15 | --- |
Component | Volume (μL) | Final |
---|---|---|
siLuc siRNA guide strand (100 μM) | 2 | 200 pmol |
[γ]-32P-ATP | 6 | 1 mCi |
SUPERase-In (40 U/μL) | 1 | 1.33 U/μL |
10x T4 PNK Buffer | 3 | 1x |
T4 PNK enzyme (10U/μL) | 3 | 1 U/μL |
Nuclease-free water | 15 | --- |