| Component | Volume (μL) | Final |
|---|---|---|
| siLuc siRNA guide strand (100 μM) | 2 | 200 pmol |
| [γ]-32P-ATP | 6 | 1 mCi |
| SUPERase-In (40 U/μL) | 1 | 1.33 U/μL |
| 10x T4 PNK Buffer | 3 | 1x |
| T4 PNK enzyme (10U/μL) | 3 | 1 U/μL |
| Nuclease-free water | 15 | --- |
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